PCR/qpcr/RT-PCR Assays

DevLab Bio can develop and optimize PCR, qPCR, RT-PCR assays for use with a wide variety of sample types, including those that perform sub-optimally with standard sample preparation procedures. Let us help you get the most from your samples!

  • Nucleic Acid Quantification
  • Genotyping & Genetic
  • Variation Analysis
  • Gene Expression
  • Genetic Variation Analysis
  • Pathogen Identification

With decades of experience in assay development, DevLab bio has an unparalleled ability for developing and optimizing customized PCR, qPCR, RT-PCR assays including, but not limited to:

DevLab bio can develop and optimize PCR/qPCR/RT-PCR assays for use with a wide variety of sample types, including those that perform sub-optimally with standard sample preparation procedures, such as:

  • Blood
  • Bone
  • Stool

pcr

PCR is a method used to amplify specific DNA sequences. PCR relies on the use of special enzymes, called polymerases, which can copy DNA strands. PCR can be used to create multiple copies of a particular DNA sequence, making it an important tool for genetic research.

PCR is often used in conjunction with other techniques, such as sequencing, to help researchers understand the function of specific genes. It can also be used to diagnose genetic disorders and to identify bacteria and viruses.

Qpcr

Quantitative PCR (qPCR) is a powerful tool used to measure the amount of target DNA in a sample. qPCR allows for the accurate and precise detection of target DNA, making it an ideal method for a variety of applications such as gene expression analysis, pathogen detection, and DNA quantification. qPCR can be used with a variety of sample types, including tissue, cells, or even body fluids. qPCR is a relatively simple assay that can be performed in any molecular biology laboratory.

Real-time PCR

PCR (polymerase chain reaction) is a method used to make multiple copies of a specific DNA sequence. Real-time PCR (RT-PCR) is a variation of PCR that can be used to measure the amount of DNA in a sample. RT-PCR is often used to detect viruses, bacteria, and other pathogens. It can also be used to measure gene expression levels.

RT-PCR works by amplifying a specific DNA sequence using special enzymes called polymerases. The amplification process is monitored in real time, allowing for the accurate measurement of the amount of DNA in the sample. RT-PCR can be used to detect very small amounts of DNA, making it an important tool in diagnosing infectious diseases.

RT-PCR can also be used to measure gene expression levels. By measuring the amount of mRNA (messenger RNA) in a sample, RT-PCR can be used to determine how much of a particular gene is being expressed. This information can be used to study the function of genes and to understand the mechanisms of disease.

All PCR services are developed under the highest quality procedures using current Good Laboratory Practices (cGLP). Whether for use in FDA 510(k) filings, clinical diagnostic use as a laboratory developed test (LDT) or for research use only, DevLab bio has the experience and knowledge to develop and validate your customized assays based on the goals of your business.